Draw A Cell That Is In An Isotonic Solution

Plasmolysis of Elodea

Authors: Laurie Aiello and Tom Schuster

Microscopy - Teacher's Guide
SED 695B; Fall 2005

Early Plasmolysis

Late Plasmolysis

Topics Addressed:

Osmosis and Diffusion
Concentration Gradients
Hypertonic, Hypotonic, Isotonic Solutions
Plant Cell Structure

Description of Investigation

Observation of a leaf of the water plant Elodea

Standards addressed:

BIOLOGY
Cell Biology
1. a. Students know cells are enclosed within semipermeable membranes that regulate their interaction with their surroundings.

SEVENTH GRADE LIFE SCIENCE
Cell Biology
1. a. Students know cells function similarly in all living organisms.
1. b. S tudents know the characteristics that distinguish plant cells from animal cells, including chloroplasts and cell walls.

under normal conditions with a microscope.

Observation of plasmolysis when the leaf is flooded with 6%

salt water.

Further observations when the plant leaf is rehydrated

with distilled water.

Materials:

Microscope
Elodea in aquarium water
Slides and Cover slips
Distilled water
6% NaCl solution (salt water)
Paper towels or tissues

Procedure:

Prepare a wet mount of one leaf from the water plant Elodea using the water in which it is kept.
Observe the cells under normal conditions, and make a sketch of what you see.
While observing the leaf under the microscope, wick a solution of 6% NaCl (sodium chloride) across the slide.
Sketch your observations.
With the same leaf, try wicking distilled water across the slide.
Record your observations.

Study Guide:

PLASMOLYSIS IN ELODEA CELLS

Questions:

Compare the location of chloroplasts in normal and plasmolyzed cells.
What was the cause for the change in the location of the chloroplasts in the two solutions?
Did the Elodea cell change shape? Why or why not?
Describe the location of the chloroplasts when the plasmolyzed cell was flooded with distilled water?
What was the cause for the change seen with distilled water?

This video shows the Elodea being "rehydrated" with distilled water. Notice that the cell wall has not changed.

The technique of "wicking" is used to draw a solution across the specimen on a slide. By placing a piece of tissue or paper towel at one edge (right) and dropping the solution at the edge of the other side (left), the solution is drawn or "wicked" across the specimen.

This picture shows plasmolyzed Elodea after the slide had been soaked with salt solution. Notice that the chloroplasts have all clumped into the middle. This demonstrates the effect on a hypertonic solution on living cells.

This picture shows normal Elodea. The chloroplasts are spread throughout the cell both before the salt solution, and after the distilled water is put onto the slide. Distilled water represents a hypotonic solution, yet the cells do not burst because of the cell wall.

References & Links:

Science NetLinks

Pleasant Grove Junior High, Utah

Wayne's World

Microscopy